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What Is Rf In Chemistry

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What Does Rf Stand For

Explain Retention Factor (Rf). | Chromatography | Analytical Chemistry

What does RF mean? This page is about the various possible meanings of the acronym, abbreviation, shorthand or slang term: RF.

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What does RF mean?

releasing factor, releasing hormone, RF
a substance produced by the hypothalamus that is capable of accelerating the secretion of a given hormone by the anterior pituitary gland

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What Is The Rf Value In Chromatography


The Rf value is defined as the ratio of the distance moved by the solute and the distance moved by the the solvent along the paper, where both distances are measured from the common Origin or Application Baseline, that is the point where the sample is

Also Know, what is retention factor in chromatography? The retention factor of a particular material is the ratio of the distance the spot moved above the origin to the distance the solvent front moved above the origin. It can be calculated using the formula: Retention factors are useful in comparing the results of one chromatogram to the results of another.

Also question is, what is the RF factor and how is it calculated?

Calculating Retention FactorWith your ruler, measure the distance the solvent traveled, which is Df, and measure the distance the test solution traveled, which is Ds. Calculate the retention factor using this equation: RF = Ds/Df. Simply divide the distance the solution traveled by the distance the solvent traveled.

What is Rf value formula?

The Rf valueThe retention factor, or Rf, is defined as the distance traveled by the compound divided by the distance traveled by the solvent. For example, if a compound travels 2.1 cm and the solvent front travels 2.8 cm, the Rf is.

What Is The Rf Value And Its Importance In Chromatography

4.8/5Rf valueChromatographyRf value

RF value The distance travelled by a given component divided by the distance travelled by the solvent front. For a given system at a known temperature, it is a characteristic of the component and can be used to identify components.

Also Know, how do you calculate the RF value What does this tell you? You divide the distance of your compounds by the distance of your solvent, and you‘ve got the Rf ratio. The farther a compound travelled, the larger it’s Rf value. Logically, you can conclude that if a compound A travels farther than compound B in a polar solvent, then it is more polar than solvent B.

Also, how is an RF value useful?

Rf value stands for the retardation factor value. It tells us how far the unknown pigment traveled in relation to the distance the solvent traveled. The Rf value is useful for scientists because it allows scientists to identify the pigment by comparing its Rf value to that of a known standard.

What is the relationship between structure and RF value?

It is a measure of how strongly the substituents are attached to the stationary phase. The stronger the adsorption, the lower is the Rf value. By the way, Rf can be calculated by dividing the distance covered by the substituent with distance covered by the mobile phase.

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How To Get Tested

Rheumatoid factor testing is ordered by a doctor. A patients doctor may suggest a rheumatoid factor test alone, but usually this test is used along with other tests to evaluate a patient for an autoimmune disorder. RF testing requires a blood sample that is normally drawn in a doctors office, hospital, or laboratory.

Why Is Rf Called Retardation Factor

Analytical chemistry

I understand that $$R_\mathrm = \frac } }$$ What I do not understand is why this is called retardation factor an analyte that travels further in the mobile phase seems to me to be less retarded by the stationary phase, while one that doesn’t travel as far seems more retarded. If we’re using this terminology why doesn’t $R_\mathrm = 1- \frac ba$?

  • 2$\begingroup$I always learned Rf as being Retention Factor, not Retardation Factor. Your question still stands though…$\endgroup$Jun 9 ’18 at 20:48
  • 2$\begingroup$They’re often used interchangeably in TLC, but IUPAC uses Retardation factor for planar chromatography , and Retention factor for column chromatography.$\endgroup$ RatharaxJun 9 ’18 at 21:01
  • $\begingroup$You have the Rf inverted. It should be a/b or distance traveled by the spot divided by distance traveled by the solvent front. Nonetheless, a low value is more retarded or retained, so your question is still a valid one. It seems to be a matter of definition.$\endgroup$

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What Is Rf In Chromatography

Homework Answers

The retardation factor, a value used in the laboratory. It’s used to measure certain properties such as polarity, or separate pigments in plants, among other things.

It’s basically a ratio of the distance traveled by the molecule of interest relative to the total distance of the solvent phase.

It’s a pretty simple concept that’s overly convoluted by some people.

What is Rf in chromatography?

Role In Chemical Separation

  • In chemical analysis: Liquid chromatography

    is performed by comparing the retardation factor of the analyte components with the retardation factors of known substances. The retardation factor is defined as the distance from the original sample spot that the component has moved divided by the distance that the mobile phase front has moved and is

  • In chromatography: Development chromatography

    reported in terms of the Rf value, the distance moved by the centre of the zone relative to the distance moved by the mobile phase front, where both are measured from the origin. Use of the solvent front as a reference point is frequently inconvenient. A standard solute is often

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Taking A Rheumatoid Factor Test

A rheumatoid factor test is performed on a blood sample that is typically taken from a vein in a patients arm. The sample of blood can be drawn in a doctors office, health clinic, or laboratory.

For an at-home RF test, a blood sample is obtained by pricking a finger and collecting the sample as instructed in a test kit.

Examples Of Radio Frequency In A Sentence

Calculating Rf (Paper Chromatography) – GCSE Chemistry Revision

radio frequency Forbesradio frequency Forbesradio frequency San Diego Union-Tribuneradio frequency Washington Postradio frequency Forbesradio frequency Anchorage Daily Newsradio frequency San Diego Union-Tribuneradio frequency Forbes

These example sentences are selected automatically from various online news sources to reflect current usage of the word ‘radio frequency.’ Views expressed in the examples do not represent the opinion of Merriam-Webster or its editors. Send us feedback.

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What Does Chromatography Involve

All types of chromatography have a stationary phase and a mobile phase. The stationary phase, as you might be able to guess from the name, does not move. In paper chromatography, the stationary phase is the paper itself.

To begin the separation of compounds, the substance to be analyzed is dotted onto the bottom edge of the piece of paper. Then the paper is placed in a beaker containing the mobile phase, which is a solvent. The solvent should be under the spot where the substance for analysis is dotted. It should not submerge the analyte.

The liquid moves up the paper by capillary action. If the analyte or part of the analyte is attracted to the mobile phase, it will move with it. If it has a stronger attraction for the stationary phase, it will not move.

After the solvent has moved up the stationary phase to some extent, you have a chromatogram. This chromatogram tells you something about the separation of different components in a mixture or tells you about what substance you have. How?

What Is Rheumatoid Factor

Rheumatoid factor is a protein made by your immune system that can attack healthy tissue in your body. Healthy people dont make RF. So, the presence of RF in your blood can indicate that you have an autoimmune disease.

Sometimes people without any medical problems produce a small amount of RF. Thats very rare, and doctors dont fully understand why it happens.

Your doctor may order a blood test to check for the presence of RF if they suspect you have an autoimmune condition, such as rheumatoid arthritis or Sjögren syndrome.

Other health problems that can cause higher-than-normal levels of RF include:

  • chronic infection
  • bone loss
  • warmth and swelling of the joints

Your doctor may also order tests to diagnose Sjögren syndrome, a condition in which your white blood cells attack the mucous membranes and moisture-secreting glands of your eyes and mouth.

The symptoms of this chronic autoimmune condition are primarily dry mouth and eyes, but they can also include extreme fatigue and joint and muscle pain.

Sjögren syndrome primarily occurs in women and sometimes appears with other autoimmune conditions, including rheumatoid arthritis.

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Where Is Chromatography Used In Real Life

Chromatography is also used to help catch criminals. In line with programmes like CSI, gas chromatography is used to analyse blood and cloth samples, helping to identify criminals and bring them to justice. Its clear to see that chromatography is an unsung hero when it comes to keeping you healthy and safe everyday.

Questions For Your Doctor About Test Results

Paper Chromatography

A patients doctor can help to address questions about rheumatoid factor testing and test results. Some questions that may be helpful to review with a doctor include:

  • How does my test result help me understand the cause of my symptoms?
  • Based on my test result, what is the likelihood that I have rheumatoid arthritis or another autoimmune disorder?
  • Do I need any follow-up tests based on my test result?

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What Does The Rf Value Tell You

The Rf values indicate how soluble the particular pigment is in the solvent by how high the pigment moves on the paper. Two pigments with the same Rf value are likely to be identical molecules. Small Rf values tend to indicate larger, less soluble pigments while the highly soluble pigments have an Rf value near to one.

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What Is A Rheumatoid Factor Test

A rheumatoid factor test measures the amount of rheumatoid factor in your blood. Rheumatoid factors are proteins produced by the immune system. Normally, the immune system attacks disease-causing substances like viruses and bacteria. Rheumatoid factors attack healthy joints, glands, or other normal cells by mistake.

An RF test is most often used to help diagnose rheumatoid arthritis. Rheumatoid arthritis is a type of autoimmune disorder that causes pain, swelling, and stiffness of the joints. Rheumatoid factors may also be a sign of other autoimmune disorders, such as juvenile arthritis, certain infections, and some types of cancer.

Other names: RF Blood Test

Purity And Separating Mixtures

Chromatograms & Calculating Rf Values | Chromatography | GCSE Chemistry (9-1) |

There are different ways to separate mixtures, for example by filtration, crystallisation, distillation, or chromatography. The method chosen depends upon the type of mixture.

is used to separate mixtures of soluble substances. These are often coloured substances such as food colourings, inks, dyes or plant pigments.

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What Does The Test Measure

Rheumatoid factor testing measures the amount of the rheumatoid factor in the blood. There are several ways to measure RF, one of which is an antibody titer test. Antibody titer tests detect how much of a specific type of antibody is present in the blood.

The development of RF and how it affects the body is not well understood. Researchers believe that rheumatoid factors may be the result of the bodys response to pathogens, like bacteria or viruses, and other triggers that confuse the immune system.

Creating Emr Vs Controlling Emr

Designing an entire RF communication system is not easy. However, it is extremely easy to generate electromagnetic radiation , and in fact you generate it even when you dont want to. Any time-varying signal in any circuit will generate EMR, and this includes digital signals. In most cases this EMR is simply noise. If its not causing any trouble, you can ignore it. In some cases it can actually interfere with other circuitry, in which case it becomes EMI .

We see, then, that RF design is not about merely generating EMR rather, RF design is the art and science of generating and manipulating and interpreting EMR in a way that allows you to reliably transfer meaningful information between two circuits that have no direct electrical connection.

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How To Calculate Rf

There are many methods to separate and identify different compounds. Chromatography is the science that allows you to separate the components of a mixture. The Rf is part of that.

There are many different kinds of chromatography. Here, the focus is the simplest kind of chromatography: paper chromatography or thin layer chromatography . Both use the same general procedure, which is described below.

Basis Of Nmr Spectroscopy


Nuclear Magnetic Resonance was first detected experimentally at the end of 1945, nearly concurrently with the work groups Felix Bloch, Stanford University and Edward Purcell, Harvard University. The first NMR spectra was first published in the same issue of the Physical Review in January 1946. Bloch and Purcell were jointly awarded the 1952 Nobel Prize in Physics for their research of Nuclear Magnetic Resonance Spectroscopy.

Nuclear magnetic resonance spectroscopy is a crucial analytical tool for organic chemists. The research in the organic lab has been significantly improved with the aid of the NMR. Not only can it provide information on the structure of the molecule, it can also determine the content and purity of the sample. Proton NMR is one of the most widely used NMR methods by organic chemists. The protons present in the molecule will behave differently depending on the surrounding chemical environment, making it possible to elucidate their structure.

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What Do The Results Mean

If rheumatoid factor is found in your blood, it may indicate:

About 20 percent of people with rheumatoid arthritis have little or no rheumatoid factor in their blood. So even if your results were normal, your health care provider may order more tests to confirm or rule out a diagnosis.

If your results were not normal, it doesn’t necessarily mean you have a medical condition needing treatment. Some healthy people have rheumatoid factor in their blood, but it’s not clear why.

Learn more about laboratory tests, reference ranges, and understanding results.

Can I Take The Test At Home

While not common, at-home testing for rheumatoid factor is available and can detect the level of RF in the blood. Taking an at-home RF test involves pricking a finger and collecting a small sample of blood. The blood sample is then sent to a laboratory for analysis and results are returned to the patient via the companys website or smartphone application.

At-home testing kits may combine an RF test with a test for cyclic citrullinated peptide antibodies. While both RF and CCP antibodies are biomarkers that may indicate a persons likelihood of having rheumatoid arthritis, neither of these tests are able to definitively diagnose the condition. Doctors use the results of these tests in combination with other factors to diagnose rheumatoid arthritis and other autoimmune disorders.

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Is There Anything Else I Need To Know About An Rf Test

An RF test is not used to diagnose osteoarthritis. Although rheumatoid arthritis and osteoarthritis both affect the joints, they are very different diseases. Rheumatoid arthritis is an autoimmune disease that affects people at any age, but usually occurs between the ages of 40 and 60. It affects more women than men. Symptoms may come and go and vary in severity. Osteoarthritis is not an autoimmune disease. It is caused by the wear and tear of joints over time and usually affects adults over the age of 65.

How Much Does The Test Cost

B.2 Calculating retention factor (Rf) values (SL)

The cost of rheumatoid factor testing depends on whether or not a patients health insurance covers this type of testing. The cost of testing can also vary based on where the test is conducted and whether RF testing is combined with other tests.

For patients with health insurance, fees for the blood draw, laboratory analysis, and office visits may be included in the cost of RF testing. A patients health insurance often covers most or all of these costs when the test is prescribed by a doctor. Its important for patients to talk to their health insurance company to understand deductibles or copays that may be required.

At-home tests may not be covered by a patients health insurance and cost around $100.

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